It depends. If there is no change in her x-ray this time, she won't be requested to do sputum test again. My dad did sputum test for the 1st time as well and they didn't require him to do sputum test for the 2nd time.Sadly my mom's medical has expired. First time, she had to do all regular tests + sputum test. She had to travel to a different region for the sputum test. Will she have to repeat sputum test again 2nd time? She is a senior widow, living alone. Due to COVID-19 in India, it will be very risky and impossible to travel to another region.